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. 2020 Nov 12;11(11):976. doi: 10.1038/s41419-020-03185-3

Fig. 5. Doxorubicin treatment induces DEPTOR expression via p53.

Fig. 5

a, b Doxorubicin treatment dramatically induced DEPTOR expression at both protein and mRNA levels. U2OS cells were treated with various genotoxic agents for 24 h and then harvested for IB with the indicated Abs a or qRT-PCR analysis b (mean ± S.E.M, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001, compared with cells treated with DMSO). BLM bleomycin, MMC mitomycin C, APH aphidicolin, HU hydroxyurea, MTX methotrexate, CTX cyclophosphamide, DOX doxorubicin, VM-26 teniposide, CPT camptothecin, PTX paclitaxel, DDP cisplatin, Act D actinomycin D, and Nut-3 nutlin-3. c The induction of DEPTOR upon doxorubicin treatment was dependent on p53. U2OS cells were transfected with the indicated siRNAs for 48 h and then treated with doxorubicin (1 μM) for the indicated time periods, followed by IB with the indicated Abs. LEX longer exposure.