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. 2020 Nov 12;11(11):976. doi: 10.1038/s41419-020-03185-3

Fig. 6. p53-induced DEPTOR expression suppresses cancer cell sensitivity to doxorubicin.

Fig. 6

a, b Deletion of p53-binding site C in the DEPTOR promoter sensitized cells to doxorubicin. U2OS cells with or without site C were treated with various concentrations of doxorubicin for 72 h and then subjected to ATPlite assay a or clonogenic survival assay b (mean ± S.E.M, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). For clonogenic survival assay, cells were seeded in triplicate in 60 mm dishes at 1000 cells (sgCtrl) or 300 cells (sgC-DEPTOR) per dish. c Deletion of p53-binding site C in the DEPTOR promoter enhanced apoptosis induced by doxorubicin via the inactivation of AKT signaling. Cells with or without site C were treated with doxorubicin (1 μM) and harvested at the indicated time points for IB with the indicated Abs. d A model of p53-mediated DEPTOR expression for the suppression of cell proliferation, survival, and chemosensitivity. Refer to the main text for details.