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. 2020 Nov 12;3:670. doi: 10.1038/s42003-020-01342-0

Fig. 3. Volatile compounds change due to interaction between B. subtilis and S. terrestris: 2-ketones induce fungal growth inhibition.

Fig. 3

a GC molecular network analysis revealed that Bs ALBA01 produces and releases a family of 2-ketones compounds, which arise as the most important volatile metabolites produced only by the bacterium. b Interaction with the fungus (ST) generates a steep drop of 2-heptanone (2H) and 2-octanone (2 O) levels in pre-ST, but not in post-ST. Both 2H and 2O are the most discriminating features between pre- and post-ST during coculture with ST. Boxplots show compound abundances, normalized by quantile normalization and auto-scaled (zero-centered and divided by SD). c Growth inhibition of S. terrestris at day 7 after inoculation of 0.02 M 2H and 0.006 M 2O on a filter paper disc placed on PDA dishes. Graphs showing the gradual reduction in mycelium growth with increasing concentrations of 2-ketones. Data shown are mean values of mycelial growth from three independent replicate experiments; red arrow indicates lethal concentration of 2H and 2O at day 7 after inoculation.