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. 2020 Oct 30;11:580997. doi: 10.3389/fneur.2020.580997

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Copyright © 2020 Wang, Zheng, Liu, Yin, Chen, Li, Li, Nie, Zeng, He, Jiang, Fang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The rat chronic post-ischemic pain model (CPIP) exhibited long-lasting bilateral mechanical allodynia. (A) Representative photographs of rat hind paw taken during CPIP model establishment, 10 min and 7 day after model establishment. The red arrow indicates the paw with the O-ring (ipsilateral side). (B) 50% paw withdraw threshold (PWT) of ipsilateral hind paw. (C) 50% PWT of contralateral hind paw. (D) Summary of the normalized area under the curve (AUC) as in (B). (E) Summary of the normalized AUC as in (C). (F) Thickness evaluation of the ipsilateral hind paw. (G) Thickness evaluation of the contralateral hind paw. n = 6 rats/group. **p < 0.01 vs. Sham group. Student's t-test or two-way ANOVA followed by Tukey post-hoc test was used for statistical analysis. *p < 0.05.