Figure 1.

NF-κB p50/p105, RELA (p65), and IκB analysis in the nucleus and cytoplasm of A. phagocytophilum-infected and uninfected HL-60 cells. The p50 component of p105 is expressed as two isoforms in infected HL-60 cells, and qualitatively lower density in uninfected than in infected cells; p50 is also detected as its precursor, p105 in both nucleus and cytoplasm (left panels). Unlike p50 and p105, RELA (p65) is qualitatively similar in uninfected and infected HL-60 cell nuclei and cytoplasm (middle panel). IκBα is also detected qualitatively at very low band density in the cytoplasm but not nuclei of uninfected, LPS-stimulated, and infected HL-60 cells as monomers; it is also detected in the nuclei of infected HL-60 cells but only as high molecular weight complexes, likely reflecting ubiquitylation. The antibody used to detect bands for each vertical nuclear (top panels) and cytoplasm (bottom panels) extract pair is labeled at the top. Jurkat cell nuclear lysate positive control = pos. The image represents a composite of six separate gel images, altered only for decreased brightness to visualize all bands, applied equally to all panels. No other manipulations were used.