Figure 3.

Prevotella isolates colonize human 3D endometrial epithelial cell model in a species-specific manner. The 3D EEC aggregates were incubated with Prevotella species at MOI 10 for 4 hours under anaerobic conditions. The SEM images depict PBS treatment and colonization with P. timonensis CRIS 5C-B1, P. disiens DNF00882, P. bivia DNF00188, P. bivia VPI6822, and P. corporis MJR7716. SEM images also demonstrated viability of Prevotella strains. Prevotella species induced species-specific microvilli remodeling and cellular secretion patterns. The images were pseudocolored using Adobe Photoshop. The images presented were selected from a minimum of 3 representative SEM images. Abbreviations: 3D, 3-dimensional; EEC, endometrial epithelial cell; MOI, multiplicity of infection; PBS, phosphate-buffered saline; SEM, scanning electron microscopy.