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. 2020 Jul 10;222(12):2041–2051. doi: 10.1093/infdis/jiaa418

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© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Induction of coxsackievirus and adenovirus receptor (CXADR) during monocyte-to-macrophage differentiation. A, Western blot analysis showing CXADR protein levels in THP-1 cells cultured as monocytes (Mo), differentiated into macrophages (M0), and further polarized into M1 or M2 macrophages. Abbreviation: MW, molecular weight. B, Immunofluorescence staining for CXADR (green) in THP-1 cells differentiated into M0, M1, or M2 macrophages. Cell nuclei were counterstained with DAPI (blue). Images were obtained with a Zeiss LSM700 confocal microscope and processed with NIS-Elements software (version 4.0). (https://www.microscope.healthcare.nikon.com/products/software/nis-elements). (Scale bar represents 10 μm.)