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. 2020 Nov 6;12:11371–11382. doi: 10.2147/CMAR.S274058

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© 2020 Liu et al.

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P-MAPK14 regulates the RUNX2 protein expression in bladder cancer cells (A) After transfected with MAPK14 siRNAs in T24 and UMUC3, MAPK14 and RUNX2 mRNA levels were determined by RT-PCR; (B) RUNX2 protein levels in T24 and UMUC3 cells were measured by Western blot; (C) After T24 and UMUC3 cells were treated with VX-702, protein levels of MAPK14, P-MAPK14 and RUNX2 were detected by Western blot; (D) MAPK14 and P-MAPK14 protein expression was detected by Western blot after overexpression of RUNX2 in T24 and UMUC3 cells; (E) The interaction between P-MAPK14 and RUNX2 was detected by Co-Immunoprecipitation assay in T24 and UMUC3 cells. (*P < 0.05, **P < 0.01, ***P < 0.001, ^nsP > 0.05).