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. 2020 Oct 30;8:588922. doi: 10.3389/fbioe.2020.588922

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Copyright © 2020 Ramos-Gomes, Ferreira, Kraupner, Alves and Markus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Uptake of NPs by monocytes and their tracking. Representative confocal images of a lung lobe from a CD68-EGFP mouse (macrophages and monocytes green). Lectin-A647 for visualization of blood vessels (red) and Ba-Cy3 NPs (yellow) were injected i.v. 5 min before sacrifice. (A) Representative frames of a time series over 4 h at an interval of 300 s showing the trajectory of individual monocytes. Scale bar represents 20 μm. (B) shows the co-localization of the Ba-Cy3 NPs with the CD68-EGFP positive monocytes within blood vessels. Images show the overlay (merged) of Lectin-A647 (red), CD68-EGFP cells (green), and Ba-Cy3 NPs (yellow). Scale bar represents 30 μm.