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. 2020 Oct 30;8:588922. doi: 10.3389/fbioe.2020.588922

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Copyright © 2020 Ramos-Gomes, Ferreira, Kraupner, Alves and Markus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tracking of the dynamic movement of tumor cells in the tumor mass. Confocal images of a lung lobe from a Nu/Nu mouse with A549-mCherry cell-induced tumors (yellow). Blood vessels were stained by i.v. injection of Lectin-A647 (red) shortly before sacrifice of the animal. (A) Images show the overlay (merged) of Lectin-A647 (red) and A549-mCherry cells (yellow). (B) Representative image of a time series over 1 h 34 min showing the trajectory of individual motile tumor cells. Scale bars represent 50 μm.