Fig. 3.

FGF2 reduced the inflammatory response in ALI. a Relative lung gene expressions of TLR4, IL-6, IL-10, CXCL1, CXCL10, MCP-1 and ICAM-1 were measured by quantitative real-time RT-PCR (n ≥ 5 per group, relative to GAPDH). b The IL-1β, IL-6, COX2, Robo4 and β-actin protein levels in the lung tissues 24 h after the surgery by western blot and the quantification of protein expressions were shown (n ≥ 3 per group). The data are represented as mean ± SEM. c The F4/80 on behalf of macrophage of lung tissue sections was stained by immunofluorescence. The sections are all shown at ×400 magnification. *P < 0.05