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. 2020 Oct 30;11:582366. doi: 10.3389/fgene.2020.582366

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Copyright © 2020 Liu, Wang, Song, Du, Yu, Zheng, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-497-5p was upregulated during osteo/odontogenic differentiation of SCAP. (A) Heat map of the differentially expressed miRNAs in differentiated SCAP. Red indicates up-regulated miRNAs and green indicates down-regulated miRNAs. (B) Expression levels of miR-376a-3p, miR-497-5p, miR-660-5p, miR-146a-5p, miR-199b-5p in differentiated SCAP examined by qRT-PCR. (C,D) The expression of osteo/odontogenic differentiation related proteins in overexpressed miR-376a-3p, miR-497-5p, miR-660-5p, miR-146a-5p, miR-199b-5p examined by western blot. (E) Time course of miR-497-5p expression during osteogenic differentiation of SCAP. Data are presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 compared with control group.