Table 2.
Baseline and hypoxic breathing parameters from Mecp2 conditional knockout and conditional rescue mice.
| CKO/CR | Cre | Genotype | N | Baseline BPM | 10% O2 Peak BPM | 10% O2 Declined BPM |
|---|---|---|---|---|---|---|
| CKO | Nestin | W | 11 | 217.39 ± 41.73 | 464.07 ± 79.87 | 230.23 ± 30.91 |
| C | 9 | 233.48 ± 35.36 | 495.70 ± 80.45 | 251.81 ± 38.92 | ||
| F | 11 | 256.67 ± 43.43 | 517.61 ± 44.85 | 301.87 ± 55.38* | ||
| K | 13 | 307.59 ± 52.13* | 482.24 ± 53.72 | 360.97 ± 63.10* | ||
| Vglut2 | W | 13 | 246.61 ± 47.30 | 539.80 ± 28.46 | 297.66 ± 50.85 | |
| C | 15 | 239.39 ± 33.31 | 524.35 ± 24.36 | 295.67 ± 51.18 | ||
| F | 14 | 264.06 ± 44.39 | 542.15 ± 26.40 | 286.42 ± 28.71 | ||
| K | 11 | 293.19 ± 30.52 | 503.16 ± 17.60* | 415.00 ± 18.42* | ||
| Th1 | W | 8 | 219.34 ± 14.79 | 499.32 ± 60.94 | 257.22 ± 39.04 | |
| C | 7 | 223.88 ± 34.53 | 549.64 ± 43.61 | 276.71 ± 36.10 | ||
| F | 8 | 237.20 ± 24.44 | 530.36 ± 61.40 | 297.02 ± 14.70 | ||
| K | 11 | 226.38 ± 34.37 | 538.47 ± 39.43 | 325.28 ± 15.42* | ||
| Viaat | W | 8 | 264.12 ± 55.73 | 520.36 ± 47.03 | 284.30 ± 21.17 | |
| C | 5 | 236.23 ± 38.06 | 497.89 ± 50.76 | 242.84 ± 26.54 | ||
| F | 7 | 273.85 ± 66.86 | 503.83 ± 53.57 | 272.89 ± 40.04 | ||
| K | 6 | 240.39 ± 23.17 | 472.51 ± 44.40 | 331.26 ± 39.22* | ||
| CR | Vglut2 | W | 6 | 219.58 ± 22.79 | 536.62 ± 48.76 | 240.16 ± 49.81 |
| C | 9 | 250.67 ± 53.49 | 531.37 ± 72.05 | 234.40 ± 31.19 | ||
| S | 5 | 299.98 ± 74.80 | 509.45 ± 61.14 | 357.19 ± 64.80* | ||
| R | 8 | 231.93 ± 34.34 | 551.62 ± 82.17 | 276.94 ± 42.65 | ||
| Th1 | W | 6 | 249.82 ± 58.56 | 516.36 ± 40.77 | 255.52 ± 26.31 | |
| C | 3 | 244.30 ± 47.40 | 418.97 ± 64.24 | 264.37 ± 42.32 | ||
| S | 7 | 251.28 ± 27.80 | 496.28 ± 44.88 | 372.93 ± 50.63* | ||
| R | 8 | 273.19 ± 61.56 | 460.77 ± 28.07 | 287.83 ± 41.26 | ||
| Viaat | W | 9 | 255.52 ± 45.10 | 468.35 ± 144.79 | 241.69 ± 28.07 | |
| C | 9 | 256.36 ± 43.87 | 462.65 ± 130.45 | 253.06 ± 35.02 | ||
| S | 7 | 273.17 ± 31.29 | 489.74 ± 38.37 | 392.88 ± 28.93* | ||
| R | 16 | 238.98 ± 44.85 | 530.35 ± 91.10 | 285.06 ± 38.56 |
Mean breath per minute (BPM) values are presented, +/– SD. *p < 0.05 effect of genotype for marked groups vs. all other groups in the same Cre-CKO/CR experiment, as determined by ANOVA and Student-Newman-Keuls post-hoc correction for multiple testing. Abbreviations, W, WT; C, CRE; F, FLOX; K, CKO; S, STOP; R, RESC. Additional parameters including Irregularity Score, Apnea Index, and uncompensated Tidal Volume were also quantified (Supplementary Table 2). Representative plethysmography traces are available (Supplementary Figure 2).