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. 2020 Oct 30;11:589364. doi: 10.3389/fmicb.2020.589364

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Copyright © 2020 Muehler, Rupp, Keceli, Brochhausen, Siegmund, Maisch, Hiller, Buchalla and Cieplik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometric measurements for membrane integrity upon treatment with aPDT using SAPYR, SA-PN-05, or CHX. Bacterial cell populations gated on dot plot FSC vs. SSC showing the chosen region of interest (ROI) for (A) A. naeslundii, (B) S. mutans, and (C) E. coli. Summarized median percentages, 1st and 3rd quartiles of unstained (gray), SG-stained (turquoise), double stained (orange) as well as PI-stained bacterial cells (red) are shown for all experimental groups (A–C). Asterisk depict statistical difference (p ≤ 0.05) among UC L- and treated groups. L- represents dark groups; L+ represents irradiated groups. n = 6.