Table 1.

Publications found in the US National Library of Medicine National Institutes of Health (NCBI-PubMed) reporting the standardisation of the loop-mediated isothermal amplification (LAMP) technique as a useful tool for diagnosing Chagas’ disease (American trypanosomiasis) in the definitive host.

Parasite	Marker	Extracted/Spiked/Natural DNA	Sample	Sample Size	Sensitivity	Reference
T. cruzi	Satellite nuclear repeat region (231 bp)	Extracted DNA	CL Brener and DM28 strains	-	CL: 5 fg DM28: 50 fg	[33]
T. cruzi	Repetitive satellite DNA sequence	Extracted, spiked and natural DNA	Reference strains belonging to the six Discrete Typing Units (DTUs) and human blood	33	Extracted DNA: ≥10−2 parasite equivalents/mL (0.3 fg) Spiked EDTA blood: 10−2 parasite equivalents/mL Spiked heparinised blood: 10−1 parasite equivalents/mL	[31]
T. cruzi	18 S rRNA	Spiked and natural DNA	Human blood	27	50 parasites/mL	[34]
T. cruzi	18 S rRNA	Spiked/natural DNA	Tulahuen strain	-	100 fg	[35]
T. cruzi	18 S rRNA	Spiked DNA	Tulahuen strain	-	1 fg	[36]