Table 3.
Parasite | Gene | Extracted/spiked/natural DNA | Sample | Sample Size | Sensitivity | Reference |
---|---|---|---|---|---|---|
E. granulosus complex | cox1 | All of them | Protoscoleces/dog faeces/faeces collected from the environment | Dog faeces: 5 Faeces collected from the environment: 61 |
10–100 fg | [42] |
E. granulosus complex | nad1 | Natural DNA | Hydatid cysts containing protoscoleces and associated germinal layers | 100 | 10 fg | [41] |
E. granulosus complex | nad1 | Diluted protoscolex or egg lysate and natural DNA | Protoscolex or eggs | 60 | 1/50 of a single protoscolex or egg | [43] |
E. multilocularis | Mitochondrial nad5 | Extracted/ natural DNA | Protoscolex tissue/dog faeces | 189 | 1 pg | [44] |
E. granulosus | Mitochondrial nad5 | Extracted and natural DNA | Isolates and faecal samples from dogs | 190 | 10 pg | [45] |
E. granulosus | Repeat region sequence | Spiked DNA | Protoscoleces recovered from affected sheep livers | - | 1 pg/200 mg faeces | [46] |