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. 2020 Oct 28;21(21):7981. doi: 10.3390/ijms21217981

Table 4.

Publications found in the US National Library of Medicine National Institutes of Health (NCBI-PubMed) reporting the standardisation of the LAMP technique as a useful tool for diagnosing foodborne trematodiases in the definitive host.

Parasite Gene Extracted/Spiked/Natural DNA Sample Sample Size Sensitivity Reference
C. sinensis cox1 All of them Human stool samples 120 1 egg/100 mg of faeces [61]
F. hepatica IGS Spiked DNA Samples collected from infected dog liver and faeces - 1 egg [62]
F. hepatica ITS2 Natural DNA Sheep faeces 15 10−3 ng [63]
F. hepatica ITS2 Natural DNA Sheep and cattle faeces Sheep:39
Cattle: 25
10−4 ng [64]
F. hepatica IGS Extracted DNA Individual worms and eggs Adults: 14
Eggs: 1
10−5 ng [65]
F. gigantica
O. viverrini Microsatellite 6 (OVMS6) Extracted DNA Adult worms 1 ng [66]
O. viverrini nad1 Natural DNA Vietnamese isolate (OvBD1) and samples collected from humans - 10−3 –10−4 ng [67]
O. viverrini ITS1 Natural DNA Adult worms from experimentally infected hamsters and children’s faeces 37 10−3 ng [68]
P. westermani Ty3/gypsy-like LTR retrotransposon (Rn1) Natural DNA Canine blood 124 2.7 fg [69]
P. westermani
(Oriental lung fluke)
ITS2 Natural DNA Adult worms from experimentally-infected dogs, eggs from patients’ sputum and pleural fluid Samples from patients’ sputum and pleural fluid: 17 10−8 ng [70]