Table 4.
Parasite | Gene | Extracted/Spiked/Natural DNA | Sample | Sample Size | Sensitivity | Reference |
---|---|---|---|---|---|---|
C. sinensis | cox1 | All of them | Human stool samples | 120 | 1 egg/100 mg of faeces | [61] |
F. hepatica | IGS | Spiked DNA | Samples collected from infected dog liver and faeces | - | 1 egg | [62] |
F. hepatica | ITS2 | Natural DNA | Sheep faeces | 15 | 10−3 ng | [63] |
F. hepatica | ITS2 | Natural DNA | Sheep and cattle faeces | Sheep:39 Cattle: 25 |
10−4 ng | [64] |
F. hepatica | IGS | Extracted DNA | Individual worms and eggs | Adults: 14 Eggs: 1 |
10−5 ng | [65] |
F. gigantica | ||||||
O. viverrini | Microsatellite 6 (OVMS6) | Extracted DNA | Adult worms | 1 ng | [66] | |
O. viverrini | nad1 | Natural DNA | Vietnamese isolate (OvBD1) and samples collected from humans | - | 10−3 –10−4 ng | [67] |
O. viverrini | ITS1 | Natural DNA | Adult worms from experimentally infected hamsters and children’s faeces | 37 | 10−3 ng | [68] |
P. westermani | Ty3/gypsy-like LTR retrotransposon (Rn1) | Natural DNA | Canine blood | 124 | 2.7 fg | [69] |
P. westermani
(Oriental lung fluke) |
ITS2 | Natural DNA | Adult worms from experimentally-infected dogs, eggs from patients’ sputum and pleural fluid | Samples from patients’ sputum and pleural fluid: 17 | 10−8 ng | [70] |