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. 2020 Oct 28;21(21):7981. doi: 10.3390/ijms21217981

Table 5.

Publications found in the US National Library of Medicine National Institutes of Health (NCBI-PubMed) reporting the standardisation of the LAMP technique as a useful tool for diagnosing foodborne trematodiases in the intermediate host.

Parasite Gene Extracted/Spiked/Natural DNA Sample Sample Size Sensitivity Reference
C. sinensis cox1 All of them Metacercariae from naturally-infected freshwater fish - 100 fg of DNA [61]
C. sinensis ITS2 Natural DNA Freshwater snails, shrimp and freshwater fish Snails: 497
Shrimp: 42
Freshwater fish: 33
10 fg (0.0002/snail) [71]
F. hepatica IGS Extracted DNA Snails Cercariae: 1 10−5 ng [65]
F. gigantica
O. viverrini nad1 Natural DNA samples collected from fish - 10−3–10−4 ng [67]
P. westermani
(Oriental lung fluke)
ITS2 Natural DNA Metacercariae from freshwater crabs and crayfish Muscle samples from infected freshwater crabs or crayfish: 35 10−8 ng [70]