Table 6.
Publications found in the US National Library of Medicine National Institutes of Health (NCBI-PubMed) reporting the standardisation of the LAMP technique as a useful tool for diagnosing HAT (sleeping sickness) in the definitive host.
| Parasite | Gene | Extracted/Spiked/Natural DNA | Sample | Sample Size | Sensitivity | Reference |
|---|---|---|---|---|---|---|
| T. brucei brucei | RIME | Spiked DNA | GVR35 strain | - | Blood treated with RBC lysis solution: 0.04 trypanosomes/mL | [22] |
| T. brucei gambiense | 5.8S ribosomal RNA -ITS2 | Extracted DNA | T. brucei gambiense DAL 972 isolate | - | ≥1 fg | [11] |
| T. brucei gambiense | TgSGP | Spiked and natural DNA | Human blood samples | - | ~1 pg (~10 trypanosomes/mL) | [77] |
| T. congolense | 18S rRNA (CON2-LAMP) | Natural DNA | Cattle blood | 420 | ND | [78] |
|
T. viva
xT. brucei rhodesiense |
Satellite DNA | |||||
| SRA | ||||||
| T. congolense | 18S rRNA (CON2-LAMP) | Natural DNA | Cattle blood | 295 | ND | [79] |
| T. vivax | Satellite DNA | |||||
| T. brucei rhodesiense | SRA | |||||
| T. brucei gambiense | TgSGP | Experimentally infected DNA | Saliva and urine from vervet monkeys | 6 | ND | [80] |
| T. brucei gambiense | RIME | Natural DNA | Human blood | 181 | ND | [81] |
| T. brucei gambiense | RIME | Natural DNA | Human blood | 253 | ND | [82] |
| T. congolense | 18S rRNA (CON2-LAMP) | Natural DNA | Canine blood | 6 | ND | [83] |
|
T. viva
xT. brucei rhodesiense |
Satellite DNA | |||||
| SRA | ||||||
| T. brucei rhodesiense | RIME SRA |
Natural DNA | Human blood and CSF | 4 | ND | [84] |
| T. brucei rhodesiense | RIME Pseudo-SRA LAMP |
Extracted DNA | T. b. rhodesiense IL1852 | - | 0.1 fg genomic DNA (0.001 parasite) | [23] |
| RIME | Spiked DNA | Human blood and CSF | - | Blood: 10 trypanosome/mL CSF: 10 trypanosomes/mL |
||
| Pseudo-SRA LAMP | Blood: 0.01 trypanosomes/mL CSF: 0.1 trypanosomes/mL |
|||||
| T. brucei | RIME | Extracted/natural DNA | T. b. rhodesiense isolate LVH 56, T. b. gambiense isolate NW2/human blood and CSF | - | 0.01 trypanosomes/mL | [85] |
| T. brucei gambiense | TgSGP | Extracted DNA | Isolate PT41 DNA | - | 10 trypanosomes/mL | [86] |
| Spiked/natural DNA | Mouse blood/human blood and CSF | 10 | ~1 trypanosomes/mL | |||
| T. brucei rhodesiense | Pseudo-SRA LAMP RIME |
Natural DNA | Human blood | 128 | ND | [87] |
| Sub-genus Trypanozoon | RIME | Extracted and Natural DNA | Human blood and CSF | 20 | 0.001 trypanosomes/mL | [27] |
| T. brucei rhodesiense | SRA | Experimentally infected/natural DNA | Mouse blood/human blood and CSF | Human sample:6 | Purified DNA: 10 trypanosomes/mL Heat-treated buffy coat: 0.1 pg (1 trypanosome/mL) a |
[88] |
|
T. brucei group T. congolense |
PFR protein A | Experimentally infected/ extracted DNA |
Mouse blood T. congolense IL-3000 |
- |
T. brucei GUTat3.1: 1 pg T. congolense: 1 ng |
[89] |
a using heat-treated buffy coat.