Table 9.
Parasite | Gene | Extracted/Spiked/Natural DNA | Sample | Sample Size | Sensitivity | Reference |
---|---|---|---|---|---|---|
Leishmania spp. | 18S rRNA | Extracted/natural DNA | Sand flies | 50 | 1 × 10−2 equivalent parasites | [100] |
L. martiniquensis | 18S rRNA | Extracted/natural DNA | Sand flies | Sand flies: 380 | 0.13 parasites/µL or 10 promastigotes in one sand fly | [104] |
L. major | 18S rRNA | Natural DNA | Sand flies | - | 101–106 Promastigotes/100 μL–0.1 parasites | [118] |
Leishmania spp. | 18S ribosomal RNA | Extracted/natural DNA | Sand flies | 397 | 0.01 parasites | [15] |