Table 15.
Publications found in the US National Library of Medicine National Institutes of Health (NCBI-PubMed) reporting the standardisation of the LAMP technique as a useful tool for diagnosing schistosomiasis in the definitive host.
| Parasite | Gene | Extracted/Spiked/Natural DNA | Sample | Sample Size | Sensitivity | Reference |
|---|---|---|---|---|---|---|
|
S. mansoni
S. intercalatum S. haematobium S. bovis |
ITS-1 | Extracted DNA | Adult worms | - |
S. mansoni and S. intercalatum: 1 pg S. haematobium: 0.1 pg S. bovis: 10 pg |
[149] |
| S. mansoni | Mitochondrial minisatellite DNA region | Extracted/natural DNA | Cutaneous and hepatic biopsies and appendix biopsy | 3 | ND | [150] |
| S. mansoni | Sm1-7 repeat fragment | Natural DNA | Human urine and stool | 111 | ND | [151] |
| S. mansoni | Mitochondrial minisatellite DNA region | Spiked/natural DNA | Human urine | 28 | 0.01 fg | [152] |
| S. haematobium | IGS | Natural DNA | Human urine | 172 | ND | [153] |
| S. mansoni | Sm1- 7 repeat fragment | Extracted/natural DNA | Human faeces | 383 | 32 fg | [154] |
| S. mansoni | Mitochondrial minisatellite DNA region | Natural DNA | Human faeces | 427 | ND | [155] |
| S. haematobium is | IGS | Spiked/natural DNA | Egyptian strain, NR-31682/human urine samples | 94 | 1 fg | [156] |
| S. japonicum | Retrotransposon SjR2 | Natural DNA | Human serum | 110 | ND | [157] |
| S. mansoni | Mitochondrial minisatellite DNA | Experimentally infected DNA | Mice faeces | - | 1 fg | [158] |
| S. japonicum | Highly repetitive retrotransposon (SjR2) | Natural DNA | Rabbit faeces | 6 | 0.08 fg | [159] |