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. 2020 Oct 29;21(21):8074. doi: 10.3390/ijms21218074

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of p53 and AKT proteins in hydroxytyrosol treated melanoma cells. A375 cells (A–C) were treated with 250 μM, 357 μM, and 500 μM of hydroxytyrosol and HT-144 cells (D–F) were treated with 250 μM, 350 μM, and 450 μM of hydroxytyrosol for 24 h and 48 h. The expression of p53, (A,D, upper panels, B,E) and AKT signalling proteins, (A,D, middle panels, C,F), was analysed in A375 and HT-144 cell lines in response to the treatment with hydroxytyrosol through western blot experiments. The equal protein loading was confirmed by the analysis of β-actin expression, (A,D, lower panels). The data are representative of several western blot experiments. The error bars indicate the standard deviation. Statistical significance was analysed by Student’s t-test: * p < 0.05 was considered significant; ** p < 0.01 highly significant; *** p < 0.001 very highly significant.

Expression of p53 and AKT proteins in hydroxytyrosol treated melanoma cells. A375 cells (A–C) were treated with 250 μM, 357 μM, and 500 μM of hydroxytyrosol and HT-144 cells (D–F) were treated with 250 μM, 350 μM, and 450 μM of hydroxytyrosol for 24 h and 48 h. The expression of p53, (A,D, upper panels, B,E) and AKT signalling proteins, (A,D, middle panels, C,F), was analysed in A375 and HT-144 cell lines in response to the treatment with hydroxytyrosol through western blot experiments. The equal protein loading was confirmed by the analysis of β-actin expression, (A,D, lower panels). The data are representative of several western blot experiments. The error bars indicate the standard deviation. Statistical significance was analysed by Student’s t-test: * p < 0.05 was considered significant; ** p < 0.01 highly significant; *** p < 0.001 very highly significant.