Figure 3.

Effects of baicalein, wogonin and oroxylin A on intracellular ROS levels and cell cycle arrest in Aβ_25–35-induced PC12 cells. The cells were pretreated with samples for 1 h, and stimulated with Aβ_25–35 for 24 h. ROS levels were observed using CM-H₂DCFDA (a) and measured by microplate reader (b). Cell cycle was measured by flow cytometry (c). Statistical analysis results of the percentage of cell cycle by flow cytometry (d). Different alphabets indicated significant difference at p < 0.05.