Table 2.
Kinetic constants for the oxidation of ABTS (λ = 660 nm; ε = 14,700 M−1 cm−1) [36] by H2O2 (2.5 mM) and hemin (2 μM) at 37 °C in the presence of varying amounts of Aβ40 or Aβ16 at pH 7.4 or 6.3. The calculated variability based on 2 independent measurements was equal to or less than 0.001 s−1.
| Species | pH 7.4−Aβ16 kobs (s−1) | pH 7.4−Aβ40 kobs (s−1) | pH 6.3−Aβ16 kobs (s−1) | pH 6.3−Aβ40 kobs (s−1) |
|---|---|---|---|---|
| Hemin | 0.013 | 0.013 | 0.016 | 0.016 |
| Aβ (1 equiv.) | 0.022 | 0.018 | 0.034 | 0.027 |
| Aβ (5 equiv.) | 0.022 | 0.021 | 0.042 | 0.033 |
| Aβ (20 equiv.) | 0.032 | 0.027 | 0.053 | 0.038 |
| Aβ (100 equiv.) | 0.054 | 0.032 | 0.092 | 0.049 |