Figure 3.

(A) In experiment 3, Tat(−) (see open bars) and Tat(+) (see hatched bars) mice were administered antalarmin (corticotrophin-releasing factor receptor antagonist; 20 mg/kg, i.p. for 6 days) and/or RU-486 (glucocorticoid receptor antagonist; i.p., 20 mg/kg, i.p. for 7 days) concurrent with the induction of HIV-1 Tat via doxycycline (30 mg/kg, i.p., once daily for 5 days with 2 days of doxycycline washout). Mice were treated with the final dose of antalarmin and/or RU-486 and then challenged with saline or oxycodone (3 mg/kg, i.p.) and assessed in an open field and light-dark transition test (n = 8–10/group). (B) Distance (m) traveled in an open field and (C) the proportional change from baseline in distance traveled in open field. (D) Circulating corticosterone and (E) the proportional change from baseline in circulating corticosterone. * indicates an interaction wherein Tat(+) mice differ from respective Tat(−) controls. † indicates an interaction wherein oxycodone-administered mice differ from respective saline-administered controls. # indicates an interaction wherein the denoted group differs from their respective vehicle controls, p < 0.05.