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. 2020 Oct 27;21(21):7996. doi: 10.3390/ijms21217996

Figure 2.

Figure 2

Screening and characterization of NbDDX3 that interacted with SDE1 from N. benthamiana. (A) NbDDX3 domain structures encoded by cDNA harbored in pGADT7. Different color boxes indicate the locations of DEAD-box helicase, RNA-binding GUCT, and zinc knuckle. (B) AH109 cells transformed with pGADT7 plasmids and pGBKT7-SDE1 grown on SD/-Ade/-Leu/-Trp/-His media supplemented with 20 μg/mL X-α- galactosidase. The interaction between VemR and RpoN2 was used as a positive control. Y2H assays were repeated three times.