Figure 2.
Screening and characterization of NbDDX3 that interacted with SDE1 from N. benthamiana. (A) NbDDX3 domain structures encoded by cDNA harbored in pGADT7. Different color boxes indicate the locations of DEAD-box helicase, RNA-binding GUCT, and zinc knuckle. (B) AH109 cells transformed with pGADT7 plasmids and pGBKT7-SDE1 grown on SD/-Ade/-Leu/-Trp/-His media supplemented with 20 μg/mL X-α- galactosidase. The interaction between VemR and RpoN2 was used as a positive control. Y2H assays were repeated three times.