Skip to main content
. 2019 May 20;157(2):173–184. doi: 10.1111/imm.13063

Figure 6.

Figure 6

Neutrophil transendothelial migration directed by CXCL8 combined with peptide. Neutrophil response to CXCL8 (10 nm), or to CXCL8 combined with each peptide, at 1–1000 nm [wild‐type (WT)/Peptide 1: KENWVQRVVEKFLKRAENS; E70K/Peptide 2: KENWVQRVVEKFLKRAKNS; or scrambled/Peptide 3: KVREKNEKWFVEQRVALNS] was measured. Cell counts were performed using counting beads by flow cytometry. Index of migrated cells or chemotaxis index (CI) is the ratio between the total number of migrated neutrophils and the number of neutrophils that migrated non‐specifically. Further titration of peptides is shown in the Supplementary material (Fig. S4). Data were analysed by one‐way analysis of variance (P < 0·0001) followed by Bonferroni post‐hoc test. ***P < 0·001 on black column indicates significant migration in response to CXCL8 compared with negative control. Data are representative of two independent experiments (n = 2) from different primary neutrophil preparations, each performed in triplicate.