TABLE 2.

tRNAs use the SID-1 and SID-2 dsRNA-specific transporters to, respectively, support imprinting inheritance and imprinting transmission via feeding.

Behavior Genotypes	Chemotaxis of naive unexposed	Imprinting after odor-exposure	Imprinting Inheritance	Imprinting after CI-RNA feeding
N2 wt	1.6 ± 0.2	2.8 ± 0.3 p < 0.001	2.8 ± 0.3 p < 0.001	2.9 ± 0.3 p < 0.001
sid-1 (qt2)	1.8 ± 0.3	3.2 ± 0.2 p < 0.001	1.7 ± 0.3 (= naive)	3.0 ± 0.2 p < 0.001
sid-1 (pk3321)	1.8 ± 0.3	2.9 ± 0.2 p < 0.001	1.8 ± 0.3 (= naive)	2.8 ± 0.3 p < 0.001
sid-2 (qt13)	0.4 ± 0.2	1.5 ± 0.2 p < 0.001	1.6 ± 0.2 p < 0.001	0.5 ± 0.2 (= naive)
	(A) Naive CI MMI	(B) CI-exposed CI MMI	(C) Unexposed F1 generation CI MMI	(D) CI-RNA fed CI MMI

Wild-type N2 larvae and larvae with the sid-1 (qt2), sid-1 (pl3321) and sid-2 (qt13) mutations were either CI 1/300 exposed, fed on 100 ng of RNA from CI 1/300 exposed N2 (CI-RNA), or unexposed as control naive. Mean Migration Indices (MMI) to CI 1/300 of naive worms (A), of CI-exposed worms (B), of unexposed F1 worms from CI-exposed (C), and of CI-RNA fed worms (D), were determined as described (experimental repeats > 4).