Fig. 2.

Current-voltage curves for 30 µM ACh-evoked responses of α7 nAChR. Averaged responses obtained during 1-second voltage ramps applied during applications of 30 µM ACh in the presence of chloride-free Ringer’s solution with either 1 (black line) or 10 mM calcium (red line). The gray and pink bands represent ± S.E.M. of the averaged data calculated at each point for the 1 and 10 mM experiments, respectively. The receptor-mediated responses were calculated by subtracting the currents produced by ramps delivered during the application of control chloride-free Ringer’s solution (either 1 or 10 mM calcium) from the responses during the voltage ramps while ACh was applied (see Materials and Methods).