Figure 2.

Relative expression of arginase in B-1 cells after intraperitoneal stimulation for 24 or 48 h with L. amazonensis promastigotes (left panel) or EVs (right panel). B-1 cells were negatively selected with anti-CD23 microbeads and positively with anti-CD19 microbeads. Animals inoculated with PBS were used as control. The RNA was extracted, the cDNA obtained, and qPCR analyses were performed to verify the arginase enzyme´s gene expression. Infection for (A) 24 h and (B) 48 h with the parasite, (C, D) stimulation for 24 or 48 h with the EVs, respectively. The bars indicate the average of triplicates, and the error bars the standard deviation. The graph is representative of three independent experiments. Test t-student, **P < 0.01; ***P < 0.001.