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. 2020 Oct 30;10:573813. doi: 10.3389/fcimb.2020.573813

Figure 3.

Figure 3

Intracellular NO production in B-1 cells after 24 or 48 h of intraperitoneal inoculation with L. amazonensis promastigotes (left panel) or EVs (right panel). (A) 24 h of infection with the parasite, (B) 48 h of stimulation with the parasite, (C) stimulation for 24 h with EVs, and (D) 48 h of stimulation with EVs. Total peritoneal cells were collected and labeled with anti-CD19 coupled with APC, anti-CD23 coupled with PE and DAF-2DA. Data acquisition was performed using a FACSCalibur cytometer. Subsequent analyzes were performed using the FlowJo software. The graphs represent the median fluorescence intensity (MFI) for the DAF probe in CD19+CD23- cells. The bars represent the mean of the duplicates, and the error bars the standard deviation. The graph is representative of two independent experiments. Test t-student, *P < 0.05; **P < 0.01, comparing stimulated (with EVs or parasites) and unstimulated B-1 cells.