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. 2020 Oct 30;10:573813. doi: 10.3389/fcimb.2020.573813

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Copyright © 2020 Reis, Dupin, Costa, Toledo, de Oliveira, Popi, Torrecilhas and Xander

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Production of ROS in B-1 cells after 24 or 48 h of stimulation with L. amazonensis promastigotes (left panel) or the parasites EVs (right panel). (A) infection for 24 h with L. amazonensis promastigotes, (B) 48 h of stimulation with the parasite, (C) 24 h of stimulation with EVs, and (D) 48 h of stimulation with EVs. Total peritoneal cells were collected and labeled with anti-CD19 coupled with APC, anti-CD23 coupled with PE, and H₂DCFHDA. Data acquisition analyzes were performed using a FACSCalibur cytometer, followed by analyzes in FlowJo software. The graphs represent the median fluorescence intensity (MFI) for the H₂DCFHDA probe in CD19⁺CD23^- gating cells. The bars represent the mean of the duplicates, and the error bars the standard deviation. The graph is representative of two independent experiments. Test t-student, **P < 0.01.