Figure 7.

Expression of lymphoid and myeloid restricted transcription factor in B-1 cells after stimulation for 24 or 48 h of intraperitoneal stimulation with L. amazonensis promastigotes (left panel) or parasite EVs (right panel). (A) infection for 24 h with the parasite, (B) stimulation for 48 h with parasite, (C) 24 h, and (D) 48 h of stimulation with EVs, respectively. B-1 cells were enriched using negative selection with anti-CD23 microbeads and positive selection with anti-CD19 microbeads. Animals inoculated with PBS were used as control. The RNA was extracted, the cDNA obtained, and qPCR analyses were performed to verify the gene expression of EBF, E2A, IL7-r, Spi1, M-csfr, and G-csfr. The bars indicate the average of triplicates and the error bars the standard deviation. The graph is representative of three independent experiments. Test t-student, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, comparing stimulated (with EVs or parasites) and unstimulated B-1 cells.