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. 2020 Oct 30;11:568694. doi: 10.3389/fimmu.2020.568694

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Copyright © 2020 Teixeira, Cavenague, Kochi, Fernandes, Souza, de Souza Filho, Vasconcellos, Heinemann and Nascimento

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of IgG isotypes produced after immunization with each recombinant protein. ELISA plates were coated with each recombinant protein and incubated with increasing concentrations of hamsters’ sera, obtained after immunizations with the respective recombinant proteins. The detection of IgG isotypes was obtained by incubation with HRP-conjugated anti-hamster IgG1 or anti-hamster IgG2/IgG3 (1:5,000) and readings were taken at 492 nm. One-way ANOVA was performed to analyze the difference between IgG1 or IgG2/3 and protein groups. For both cases significance was <0.05.