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. 2020 Oct 30;11:568694. doi: 10.3389/fimmu.2020.568694

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Copyright © 2020 Teixeira, Cavenague, Kochi, Fernandes, Souza, de Souza Filho, Vasconcellos, Heinemann and Nascimento

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Survival curves of immunized hamsters after challenge with Leptospira interrogans. Virulent leptospires (10⁴) were inoculated intraperitoneally in hamsters, previously immunized with recombinant proteins, and monitored daily for 21 days, inspecting the clinical signs of leptospirosis. Animal groups immunized with PBS (control) and bacterin were used as negative and positive controls, respectively. The graphs illustrate the survival curves obtained for Lsa25.6, Lsa16, LipL46, and Lsa14 (A) and Lsa19, rLIC11711, Lsa24.9, and rLIC13259 (B) in two independent experiments (left and middle) and the average result (right).