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. 2020 Oct 30;11:568694. doi: 10.3389/fimmu.2020.568694

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Copyright © 2020 Teixeira, Cavenague, Kochi, Fernandes, Souza, de Souza Filho, Vasconcellos, Heinemann and Nascimento

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protective effect elicited in hamster by recombinant proteins assessing the presence or absence of leptospires in animal kidneys after the challenge. Kidneys were removed from euthanized hamsters in 21 days-post challenges with Leptospira interrogans and the resulting macerate was incubated in liquid Ellinghausen-McCullough-Johnson-Harris (EMJH) medium and monitored for 30 days. The presence or absence of leptospires in cultures was determined as positive and negative culture, respectively. The graph represents the percentages obtained of two experiments.