Figure 3.

UV chromatogram of the E3 extract from Platycodon grandiflorum A. DC recorded at λ = 230 nm (A) nm and λ = 280 nm (B) by UPLC system. Chromatographic profiles of metabolites were obtained with gradients of two solvents: A (water acidified with 0.1% formic acid) and B (100% acetonitrile), mobile phase flow of 0.4 mL/min on a RP C18 column at 40 °C. The gradient elution: 100% of A linearly changed to 80% of A over 2 min, then to 70% of A over 8 min and to 5% of A over 1 min. The number corresponded to numbers of identified metabolites in Table 2.