Table 7.

Real-time PCR conditions for particular genes.

Gene	Number of Cycles	Initial Denaturation	Denaturation	Annealing	Elongation
GAPDH	35	95 °C, 10 min	95 °C, 4s	56 °C, 4s	72 °C, 8s
TGF-β1	40	95 °C, 10 min	95 °C, 10s	58 °C, 6s	72 °C, 10s
TGF-β2	40	95 °C, 10 min	95 °C, 10s	60 °C, 7s	72 °C, 10s
TGF-β3	40	95 °C, 10 min	95 °C, 10s	60 °C, 7s	72 °C, 10s
Mucin (Muc5AC)	40	95 °C, 10 min	95 °C, 10s	60 °C, 7s	72 °C, 10s
VEGF	40	95 °C, 10 min	95 °C, 10s	60 °C, 7s	72 °C, 10s