Table 2.
Motifs containing known functional residues in α-CAs. Motif residues displayed are from Persephonella marina (PmCA), and those in bold and underlined have been identified as functionally important.
| Motif | E-Value | Residues | Function |
|---|---|---|---|
| 1 | 1.5 × 10−251 | YYRYSGSLTTPPCSEGVRWI |
Cys197—intra-subunit disulfide Cys [29,34,74,75,76] Leu192, Val201 & Trp203—CO2 binding pocket residues [29,37,75] Thr193 & Thr194—proton shuttling residues [29,34] Arg187, Tyr188, Ser189, Glu199, Gly200, Val201 & Arg202—interface residues (present study) |
| 2 | 9.9 × 10−180 | QFHFHAPSEHKLKGQHYPFE | His107 & His109—Zn2+ coordinating residues [30,31,32] |
| 3 | 1.6 × 10−146 | MGGDTNRPVQPLNARMIME | Asn236, Ala237, Arg238, Met239 & Met241—interface residues (present study) |
| 4 | 7.8 × 10−146 | AHFVHADKHGNLAVIGVFFK |
His126—Zn2+ coordinating residues [30,31,32] Val128 & Val138—CO2 binding pocket residing residues [29,34,37,75] Asn135—interface residue (present study) |
| 5 | 2.4 × 10−119 | YHGEHGPEHWGDLKDEYIMC |
Tyr25—proton shuttling residue [29,34] Asp39, Ile42 and Met43—interface residues (present study) Cys44—intra-subunit disulfide Cys residue [29,34,74,75,76] |
| 6 | 3.3 × 10−110 | NNGHTIKVSYEPGSYIVVDG |
Asn80, His82 & Lys85—proton shuttling residues [29,34,77] Asp97—interface residues (present study) |
| 7 | 7.2 × 10−77 | KNQSPVDINRIVDAKLKPIK | Lys48, Asn49, Arg57, Val59, Asp60, Ala61, Lys62 & Leu63—interface residues (present study) |