Figure 1.

BMP-2, but not FGF-2, promotes repair of mouse calvarial defect. Collagen pellets containing BMP-2 (10 µg) or FGF-2 (1 µg, 10 µg) or distilled water (DW, control) were transplanted into mouse calvarial defects. (A) Frontal section (upper panel) and 3D (lower panel) images of micro-CT, 14 days after transplantation. (B) Graph shows the quantitative analysis of the regenerated bone volume (RBV) (n = 3, *** p < 0.001 versus control pellet (#). One-way ANOVA/Tukey). (C) HE-staining of frontal sections at the defect area at 5 days and 14 days after surgery. The results are representative of at least three independent experiments. Lower panels are high magnification images of the squares in the upper images. Two-way arrows, white arrowheads and black arrowheads indicate the bone defects, newly-formed bone and calvarial bone, respectively.