Table 1.
Antibodies used for flow cytometry.
| Antibody | Conjugation | Clone | Source | Cat.# |
|---|---|---|---|---|
| CD3e | APC | 145-2C11 | BioLegend | 100312 |
| CD11b | APC | M1/70 | BioLegend | 101212 |
| CD16/32 | 2.4G2 | BD Biosciences | 553142 | |
| CD31 | APC | MEC13.3 | BioLegend | 102509 |
| CD31 | PE | MEC13.3 | BioLegend | 102507 |
| CD45 | APC | 30-F11 | BioLegend | 103111 |
| CD45R (B220) | APC | RA3-6B2 | BioLegend | 103211 |
| CD48 | BV421 | HM48-1 | BioLegend | 103428 |
| CD117 (c-Kit) | FITC | 2B8 | BioLegend | 105806 |
| CD150 | PE | TC15-12F12.2 | BioLegend | 115903 |
| Leptin Receptor | Biotin | R&D systems | BAF497 | |
| Lineage marker cocktail | APC | 145-2C11(CD3e), M1/70(CD11b), RA3-6B2 (CD45R/B220), TER-119 (Ly76), RB6-8C5 (Ly6G/C) | BD Biosciences | 51-9003632 |
| Ly-6A/E (Sca-1) | PE-Cy7 | D7 | BioLegend | 108114 |
| Ly-6G/C (Gr-1) | BV421 | RB6-8C5 | BD Biosciences | 562709 |
| Streptavidin | BV421 | BioLegend | 405226 | |
| Ter119 | APC | TER-119 | BioLegend | 116211 |
| 7-AAD | BioLegend | 420404 |