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siRNA-mediated HJURP depletion in colorectal cancer cell lines. Colorectal cancer cell lines including HT29, HCT116, SW480, and SW620 were transduced with either control or HJURP siRNA. (A) RT-PCR was performed to determine HJURP mRNA expression cancer cell lines. (B) Immunoblot with anti-HJURP antibody was conducted to analyzed HJRUP protein level. GAPDH and β-actin was used as the loading control for RT-PCR and immunoblot.
