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. 2020 Oct 26;21(21):7942. doi: 10.3390/ijms21217942

Figure 6.

Figure 6

mRNA levels of Napepld, coding for NAPE-hydrolysing phospholipase D (NAPE-PLD) in J774 cells cultured in 24-well plates for 24 h and treated for 2, 4, and 6 h with lipopolysaccharide (LPS, 0.1 µg/mL) + interferon-γ (100 U/mL) and/or 10 µM PEA. Shown are box and whisker plots, N = 8. The mRNA values are determined using Rpl19 as reference gene and are given as ∆Ct with the mean values relative to the unstimulated controls at the 2 h time point on the right y-axis. A decrease of 1 ∆Ct unit represents a doubling in the mRNA concentration. The box shows the results of a 3-way ANOVA (ti, time; L, LPS + interferon-γ; P, PEA. These indicate a time-dependent effect of the inflammatory stimulus on Napepld expression, but no significant effect of PEA treatment. Figure redrawn from [87].