Figure 3.
PAK1 knockout blocked the inhibition of cannabis oil on pancreatic tumour growth. Murine pancreatic cancer (PC) cells were subcutaneously injected into the flanks of PAK1 wild type (WT, n = 10) and knockout (KO, n = 9) male mice (8-week old). After 5 days of tumour cell injection and when the average tumour volumes reached 50 mm3, cannabis oil (Can-oil) containing CBD and THC with 1:1 ratio, was given orally every day for 8 days. Tumour volumes (A) were measured daily and tumour weight (B) was determined at the end of the experiment. (C): photos of tumors dissected at the end of the experiment. CT: control, Can: cannabis oil, Can-oil: cannabis oil. *, p < 0.05 compared to non-treated PAK1 WT control.