Skip to main content
. 2020 Oct 31;21(21):8184. doi: 10.3390/ijms21218184

Figure 2.

Figure 2

Effect of verteporfin on UUO-induced renal fibroblast activation. Representative immunofluorescence staining of α-SMA (A) and FPS-1 (B) from the kidneys of sham- and UUO-operated mice treated with vehicle (Veh) or verteporfin (VP). The nuclei were stained with DAPI. The bar graph shows the number of α-SMA and FPS-1 positive cells from ten randomly chosen, non-overlapping fields (n = 10) at a magnification of x400 (n = 15/group). Scale bar = 50 µm. Data are expressed as the mean ± SD. *** p < 0.001 versus Veh or VP; # p < 0.05, ## p < 0.01 versus UUO. α-SMA (C) and Type I collagen (D) expression in kidney tissue from sham and UUO-operated mice treated with Veh or VP was evaluated by Western blotting. Data from the densitometric analysis are presented as the relative ratio of each protein to GAPDH. The relative ratio measured in the kidneys from sham-operated mice treated with Veh is arbitrarily presented as 1. Data are expressed as the mean ± SD. # p < 0.05 versus UUO. Veh, vehicle; VP, verteporfin; UUO, unilateral ureteral obstruction operated mice; α-SMA, α-smooth muscle actin; FSP-1; fibroblast-specific protein-1; DAPI, 4’,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.