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(a) DBCO-C2TCO-AF594 (21) as a click- and cleavable fluorescent tag for conjugation with azide-labeled antibodies. (b) Staining of EGFR-positive A431 cells with Cetux-C2TCO-AF594 (ON-state) and subsequent cleavage with HPA (19) (OFF-state). (c) Cell imaging confirmed near-quantitative bioorthogonal disassembly of Cetux-C2TCO-AF594 within 6 min.
