Figure 6.

PP2 inhibits Src and Fyn kinase expression in LPS-induced AKI mice. Six-week-old male C57/BL6 mice were pretreated with PP2 (2 mg/kg) for 2 h and then administered LPS (15 mg/kg) for 18 h. (A–H) Immunoblotting analysis of phosphorylated/total Fyn and Src kinase in the kidney. Levels of proteins were normalized to their respective total protein. (I) Kidney sections were subjected for immunofluorescence staining with phospho-Fyn (red) and phospho-Src (green) antibodies and 4’,6-diamidino-2-phenylindole (DAPI; blue). Data are presented as mean ±SE of 6–8 mice. * p < 0.05 vs. control, ^† p < 0.05 vs. LPS.