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. 2020 Oct 30;11:561537. doi: 10.3389/fphar.2020.561537

FIGURE 2.

FIGURE 2

Ecdysterone alters the cell cycle and induces cell death depending on the cancer cell line. (A) Cell cycle profiles (flow cytometry) of breast cancer cell lines treated with 0, 250, 500 or 750 µM Ecdy for 48 h. Cell cycle analyses are shown for MCF7 (B), MDA-MB-231 (C), and MDA-MB-468 (D) cells. Results are displayed as the mean ± SEM of three experiments. *p < 0.05; **p < 0.01. (E) Annexin V-FITC/Propidium iodide (PI) profile of the same breast cancer cell lines treated with 0, 250, 500 or 750 µM Ecdy for 48 h (flow cytometry). Rates of cell death related to DMSO-treated cells (0 µM Ecdy) calculated for (F) MCF7, (G) MDA-MB-231, and (H) MDA-MB-468 cells are represented as diagrams. Results are calculated as mean ± SEM of three experiments. *p < 0.05; **p < 0.01; n.s – not significant.

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