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Ecdysterone synergizes with doxorubicin to induce apoptosis in MDA-MB-468 cells. Confocal microscopy showing an increase in cytochrome C release upon combined treatment with doxorubicin and Ecdy. MDA-MB-468 cells treated with 0.15 µM doxorubicin or 250–750 µM Ecdy for 48 h alone or in combination were stained with DAPI and anti-cytochrome C antibodies and subsequently analyzed by confocal microscopy.
