Table 2.
Induction of apoptosis in HCT-116 and SW620 cells after treatment with Agarikon Plus (AP), Agarikon.1 (AG.1) or their combination 1:1 (AP + AG.1) a.
| Cell Line | Duration of Treatment | Concentration | Early Apoptosis (%) | Late Apoptosis (%) | Necrosis (%) |
|---|---|---|---|---|---|
| HCT-116 | 24 h | Control | 7.2 ± 0.7 | 2.46 ± 1.09 | 3.17 ± 1.01 |
| AP c1 | 16.24 ± 2.7 | 4.12 ± 1.01 | 2.34 ± 1.06 | ||
| AP c2 | 8.34 ± 1.88 | 3.3 ± 1.21 | 5.07 ± 2.42 | ||
| AP c3 | 5.7 ± 0.47 | 3.24 ± 1.81 | 3.36 ± 0.92 | ||
| AG.1 c2 | 44.3 ± 8.5 ** | 10.1 ± 0.51 ** | 0.82 ± 0.81 | ||
| AG.1 c3 | 21.01 ± 5.97 | 11.78 ± 2.55 ** | 2.44 ± 2.25 | ||
| AP + AG.1 c1 | 46.56 ± 14.8 ** | 4.5 ± 1.62 | 0.55 ± 0.53 | ||
| AP + AG.1 c2 | 23.6 ± 3.3 | 3.04 ± 0.66 | 2.04 ± 0.42 | ||
| AP + AG.1 c3 | 13.71 ± 6.03 | 5.8 ± 2.33 | 4.17 ± 1.45 | ||
| SW620 | 24 h | Control | 5.23 ± 1.6 | 2.22 ± 1.22 | 1.7 ± 1.43 |
| AP c1 | 14.57 ± 1.3 | 2.62 ± 0.63 | 0.81 ± 0.58 | ||
| AP c2 | 15.26 ± 1.83 | 3.53 ± 0.68 | 1.41 ± 0.6 | ||
| AP c3 | 5.69 ± 0.86 | 3.34 ± 0.53 | 1.97 ± 0.82 | ||
| AG.1 c2 | 50.31 ± 5.05 *** | 4.48 ± 1.59 | 0.19 ± 0.28 | ||
| AG.1 c3 | 30.05 ± 4.32 | 4.83 ± 1.59 | 0.66 ± 0.94 | ||
| AP + AG.1 c1 | 45.98 ± 5.08 *** | 6.94 ± 2.63 ** | 0.79 ± 0.98 | ||
| AP + AG.1 c2 | 31.07 ± 2.56 | 4.71 ± 0.96 | 0.49 ± 0.25 | ||
| AP + AG.1 c3 | 9.34 ± 0.92 | 3.74 ± 0.63 | 2.72 ± 0.2 |
a Induction of apoptosis was analyzed after Annexin V and PI staining protocol for flow cytometry. The cells were sorted into intact cells (Annexin V−PI−), early apoptotic cells (Annexin V+ PI−), late apoptotic cells (Annexin V+PI+), and necrotic cells (Annexin V−PI+). Values are the means ± SD of three independent experiments. * Significantly different ** p < 0.01; *** p < 0.001) in comparison with control group. The following dilutions were applied: c1 (100×); c2 (1000×); c3 (10,000×). The values for AG.1 c1 could not be read due to precipitate formation. AP, Agarikon Plus; AG.1, Agarikon.1; 5-FU, 5-fluorouracil.